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(A) Concentration-response curves <t>for</t> <t>endothelium-dependent</t> relaxation to acetylcholine (ACh, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (B) Maximal relaxation (Emax) to ACh. (C) Concentration-response curves for endothelium-independent relaxation to sodium nitroprusside (SNP, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (D) Maximal relaxation (Emax) to SNP. Relaxation is expressed as the percentage of phenylephrine (PE, 10 μM)-induced pre-contraction. Data are presented as mean ± SD (n = 3 per group). Statistical analysis was performed using one-way ANOVA (for Emax) or two-way repeated-measures ANOVA (for curves) followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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(A) Concentration-response curves <t>for</t> <t>endothelium-dependent</t> relaxation to acetylcholine (ACh, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (B) Maximal relaxation (Emax) to ACh. (C) Concentration-response curves for endothelium-independent relaxation to sodium nitroprusside (SNP, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (D) Maximal relaxation (Emax) to SNP. Relaxation is expressed as the percentage of phenylephrine (PE, 10 μM)-induced pre-contraction. Data are presented as mean ± SD (n = 3 per group). Statistical analysis was performed using one-way ANOVA (for Emax) or two-way repeated-measures ANOVA (for curves) followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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(A) Concentration-response curves <t>for</t> <t>endothelium-dependent</t> relaxation to acetylcholine (ACh, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (B) Maximal relaxation (Emax) to ACh. (C) Concentration-response curves for endothelium-independent relaxation to sodium nitroprusside (SNP, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (D) Maximal relaxation (Emax) to SNP. Relaxation is expressed as the percentage of phenylephrine (PE, 10 μM)-induced pre-contraction. Data are presented as mean ± SD (n = 3 per group). Statistical analysis was performed using one-way ANOVA (for Emax) or two-way repeated-measures ANOVA (for curves) followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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(A) Concentration-response curves for endothelium-dependent relaxation to acetylcholine (ACh, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (B) Maximal relaxation (Emax) to ACh. (C) Concentration-response curves for endothelium-independent relaxation to sodium nitroprusside (SNP, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (D) Maximal relaxation (Emax) to SNP. Relaxation is expressed as the percentage of phenylephrine (PE, 10 μM)-induced pre-contraction. Data are presented as mean ± SD (n = 3 per group). Statistical analysis was performed using one-way ANOVA (for Emax) or two-way repeated-measures ANOVA (for curves) followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: PLOS One

Article Title: NOX2 inhibition attenuates oxidative stress and eNOS uncoupling in pulmonary arteries of rats following simulated air diving

doi: 10.1371/journal.pone.0351145

Figure Lengend Snippet: (A) Concentration-response curves for endothelium-dependent relaxation to acetylcholine (ACh, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (B) Maximal relaxation (Emax) to ACh. (C) Concentration-response curves for endothelium-independent relaxation to sodium nitroprusside (SNP, 10 ⁻ ⁹–10 ⁻ ⁴ M) in isolated pulmonary artery rings from Group C, Group D, and Group D + GSK2795039. (D) Maximal relaxation (Emax) to SNP. Relaxation is expressed as the percentage of phenylephrine (PE, 10 μM)-induced pre-contraction. Data are presented as mean ± SD (n = 3 per group). Statistical analysis was performed using one-way ANOVA (for Emax) or two-way repeated-measures ANOVA (for curves) followed by Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Subsequently, endothelium-dependent relaxation was induced by cumulative addition of acetylcholine (ACh, 10 − 9 to 10 − 4 M; MedChem Express, NJ, USA; Cat. No. HY-B0282).

Techniques: Concentration Assay, Isolation